BIO 465L - Stem Cell Biology Lab (1)

This course provides the students hands-on experience with culturing stem cells and non-stem cells and comparing their differentiation capacity. It also provides the students an opportunity to come up with their own scientific hypotheses and design the experimental solutions for those hypotheses based on knowledge obtained from both BIO 465 and BIO 465L. Topics include: 1) Basic aseptic cell culture skills, cell expansion, freezing, thawing and transfection; and 2) Differentiation capacity comparison between lineage committed non-stem cells and adult stem cells based on adipogenic differentiation (fat cell formation) and osteogenic differentiation (bone cell formation). The adult stem cells we use are commercially available and isolated from human bone marrow or adipose tissue.

Prerequisite(s): BIO 310.
Pre or Corequisite(s): BIO 465.
Component(s): 1 three-hour laboratory.
When Offered: F, Sp
Faculty: Zhao.